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This extra calcium chloride triggers the platelets to release at least 7 different growth factors that then trigger the unipotent stem cells to grow younger tissue--activating unipotent stem cells. The doctor injects the PRP into an area called the O-Spot--a collection of structures that activate the orgasm system. The woman usually enjoys the effects of the O-Shot™ almost immediately as the growth factors begin to rejuvenate and enhance the sexual response. So, the O-Shot® (or Orgasm Shot®) is a nonsurgical procedure that uses the growth factors each woman has in her own body to stimulate vaginal and clitoral rejuvenation to activate the Female Orgasm System. Thus far, almost all women receiving O-Shot™ procedure enjoy an increase in their sexual response, and for many the increase is dramatic. These sometimes experience other effects listed here: