g) Using a value of x 104 L/mol for the association constant of albumin for T, the calculated albumin-bound T varied from nmol/L (40 g/L albumin) to nmol/L (50 g/L albumin). In view of the relatively unimportant changes in FT, when the albumin concentration varies by as much as 25%, it was concluded that for routine purposes FT could be calculated assuming an albumin concentration of 43 g/L ( x 10-4 mol/L) if one is not dealing with sera from patients with marked abnormalities in plasma protein composition, such as in nephrotic syndrome or cirrhosis of the liver, or with sera obtained during pregnancy, in which cases the actual albumin concentration should be taken into account.
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Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In wom-en, high levels of testosterone are generally found in hirsutism and virilization, poly-cystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: hy-popituitarism, Klinefelter's syndrome, testicular feminization, Orchidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases.